https://ogma.newcastle.edu.au/vital/access/ /manager/Index en-au 5 Comparative bioremediation of petroleum hydrocarbon-contaminated soil by biostimulation, bioaugmentation and surfactant addition https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:15822 Wed 11 Apr 2018 09:51:36 AEST ]]> Bioavailability of weathered hydrocarbons in engine oil-contaminated soil: impact of bioaugmentation mediated by Pseudomonas spp. on bioremediation https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:32271 -1 soil). Both the bacterial strains exhibited a great potential in remediating weathered hydrocarbons of engine oil. Addition of inorganic fertilizers (NPK), at recommended levels for bioremediation, resulted in significant inhibition in biostimulation/enhanced natural attenuation as well as bioaugmentation. The data on dehydrogenase activity clearly confirmed those of bioremediation strategies used, indicating that this enzyme assay could serve as an indicator of bioremediation potential of oil-contaminated soil. Extraction of TPHs from engine oil-contaminated soil with hydroxypropyl-ß-cyclodextrin (HPCD), but not 1-butanol, was found reliable in predicting the bioavailability of weathered hydrocarbons. Also, 454 pyrosequencing data were in accordance with those of bioremediation strategies used in the present microcosm study, suggesting the possible use of pyrosequencing in designing approaches for bioremediation.]]> Thu 17 May 2018 13:51:27 AEST ]]> Bioaugmentation with novel microbial formula vs. natural attenuation of a long-term mixed contaminated soil - treatability studies in solid- and slurry-phase microcosms https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:24350 -1 of total PAHs using novel acid-, metal-tolerant, N-fixing, P-solubilizing, and biosurfactant-producing LMW and HMW PAH-degrading bacterial combinations as inoculums was compared in slurry- and solid-phase microcosms over natural attenuation. Bioaugmentation of 5 % of bacterial consortia A and N in slurry- and solid-phase systems enhanced 4.6-5.7 and 9.3-10.7 % of total PAH degradation, respectively, over natural attenuation. Occurrence of 62.7-88 % of PAH biodegradation during natural attenuation in soil and slurry illustrated the accelerated rate of intrinsic metabolic activity of the autochthonous microbial community in the selected MGP soil. Monitoring of the total microbial activity and population of PAH degraders revealed that the observed biodegradation trend in MGP soil resulted from microbial mineralization. In the slurry, higher biodegradation rate constant (k) and lower half-life values (t1/2) was observed during bioaugmentation with consortium N, highlighting the use of bioaugmentation in bioslurries/bioreactor to achieve rapid and efficient bioremediation compared to that of a static solid system. In general, natural attenuation was on par with bioaugmentation. Hence, depending on the type of soil, natural attenuation might outweigh bioaugmentation and a careful investigation using laboratory treatability studies are highly recommended before the upscale of a developed bioremediation strategy to field level.]]> Sat 24 Mar 2018 07:10:17 AEDT ]]> Rhizoremediation as a green technology for the remediation of petroleum hydrocarbon-contaminated soils https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:38496 Mon 11 Oct 2021 15:51:00 AEDT ]]>